Revista de Investigaciones Altoandinas - Journal of High Andean Research

Vol. 21 No. 2 (2019)
Original articles

Effect of plant growth regulators in the in vitro multiplication and rooting of senecio calvus (Asteraceae), high-andean medicinal plant, endemic to Peru

PDF (Spanish)
  • Yenilda Laguna-Ibarra,
  • Jhonny Cueva-López,
  • Carmen Tamariz-Angeles,
  • Percy Olivera-Gonzales
Yenilda Laguna-Ibarra
Universidad Nacional Santiago Antúnez de Mayolo, Huaraz
Jhonny Cueva-López
Universidad Nacional Santiago Antúnez de Mayolo, Huaraz
Carmen Tamariz-Angeles
Universidad Nacional Santiago Antúnez de Mayolo, Huaraz
Percy Olivera-Gonzales
Universidad Nacional Santiago Antúnez de Mayolo, Huaraz

Published 2020-08-28

Keywords

  • gibberellic acid,
  • naphthaleneacetic acid,
  • Asteraceae,
  • benzylaminopurine,
  • in vitro culture

Copyright (c) 2019 Yenilda Laguna-Ibarra, Jhonny Cueva-López, Carmen Tamariz-Angeles, Percy Olivera-Gonzales

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This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Laguna-Ibarra, Y., Cueva-López, J., Tamariz-Angeles, C., & Olivera-Gonzales, P. (2020). Effect of plant growth regulators in the in vitro multiplication and rooting of senecio calvus (Asteraceae), high-andean medicinal plant, endemic to Peru. Revista De Investigaciones Altoandinas - Journal of High Andean Research, 21(2), 111-121. https://doi.org/10.18271/ria.2019.455

Abstract

In vitro propagation of Senecio calvus was carried out as an alternative for its conservation. The buds were disinfected with HgCl2 (0.01 and 0.1%) with and without periodic addition of antibiotics. Murashige and Skoog culture medium (1/2) plus 2% sucrose and 0.3% phytagel 0.3%, and pH 5.7 was supplemented with benzylaminopurine for multiplication; while for the rooting naphthaleneacetic acid or gibberellic acid were used. Treatments without growth regulator were added. For acclimation two natural substrates were evaluated. The highest percentage of disinfection was obtained with 0.1% HgCl2 for 5 minutes adding rifampicin (72 μg·mL-1) and nystatin (200 μg·mL-1) every three days per 18 days. Benzylaminopurine increased the survival of the introduced buds and the percentage of shooted plants; however the number of shoots per plant did not show significant differences. Gibberellic acid had a positive effect on rooting while naphthaleneacetic acid induced callusing formation. The best substrate for acclimatization was soil + moss (1: 1).

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References

  1. Ahsan, T., Amjad, N., Iqbal, A., & Javed, A. (2013). A review: tissue culturing of important medicinal plants. International Journal of Water Resources and Environmental Sciences, 2 (4), 76-79. doi: 10.5829/idosi.ijwres.2013.2.4.11120
  2. Alvarado, M. A., & Solano, J. A. (2002). Producción de sustratos para viveros. Costa Rica: Editorial Proyecto regional de Fortalecimiento de la Vigilancia fitosanitaria en cultivos de exportación no tradicional-VIFINEX. Recuperado de http://www.cropprotection.es/documentos/Compostaje/Sustratos-para-Viveros.pdf
  3. Beltrán, H., & Roque J. (2015). El género Senecio L. (Asteraceae-Senecioneae) en el Departamento de Lima, Perú. Arnaldoa, 22 (2), 395-412.
  4. Bisht, T. S., Rawat, L., Chakraborty, B., & Yadav, V. (2018). A recent advances in use of plant growth regulators (pgrs) in fruit crops - a review. International Journal of Current Microbiology and Applied Sciences, 7 (5), 1307-1336. https://doi.org/10.20546/ijcmas.2018.705.159
  5. Carraz, M., Lavergne, C., Jullian, V., Wright, M., Gairin, J. E., Gonzales de la Cruz, M., & Bourdy, G. (2015). Antiproliferative activity and phenotypic modification induced by selected Peruvian medicinal plants on human hepatocellular carcinoma Hep3B cells. Journal of Ethnopharmacology, 166, 185-199. doi: https://doi.org/10.1016/j.jep.2015.02.028
  6. Cob-Uicab, J.V., Sabja, A.M., Ríos-Leal, D., Lara-Aguilar, A., Donoso, P., Gonzáles, M., & Escobar, B. (2011). Potencial de la organogénesis como estrategia para la masificación in vitro de Fitzroya cupressoides en Sudamérica Austral. Revista Chapingo Serie Ciencias Forestales y del Ambiente, 17 (3), 423-433. Recuperado de http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S2007-40182011000300012
  7. Cristea, V., Brummer, A.T., Jarda, L., & Miclăuş, M. (2010). In vitro culture initiation and phytohormonal influence on Dianthus henteri – a Romanian endemic species. Romanian Biotechnological Letters, 15 (1), 25-33. Recuperado de https://www.rombio.eu/rbl1vol15Supplement/4%20Victoria%20Cristea.pdf
  8. Cruz-Rosero, N., Canchignia-Martínez, H., Morante-Carriel, J., Nieto-Rodríguez, E., Cruz-Rosero, E., & Cabrera-Casanova, D. (2016). In vitro propagation of the Orito banana cultivar (Musa acuminata AA). Biotecnología Aplicada, 33 (4), 4201-4204. Recuperado de http://www.medigraphic.com/pdfs/biotecapl/ba-2016/ba164a.pdf
  9. Ford, Y. Y., Taylor, J. M., Blake, P. S., & Marks, T. R. (2002). Gibberellin A3 stimulates adventitious rooting of cuttings from cherry (Prunus avium). Plant Growth Regulation, 37 (2), 127-133. doi: 10.1023/A:1020584627919
  10. George, E. F., Hall, M. A., & Klerk, J. D. (2008). Plant growth regulators I: Introduction; auxins, their analogues and inhibitors. In: E. F. George, M. A. Hall & G.J. Klerk Ed.), Plant propagation by tissue culture. (pp. 175–204). Dordrecht, Netherlands: Springer. Recuperado de https://investigacionfitopatologiaumar.files.wordpress.com/2016/06/plant-propagation.pdf
  11. De la Cruz, M. G., Malpartida, S.B., Beltrán, H.S., & Jullian, V. (2014). Hot and cold: Medicinal plant uses in quechua speaking communities in the high andes (Callejón de Huaylas, Ancash, Perú). Journal of Ethnopharmacology, 155, 1093-1117. doi:http://dx.doi.org/10.1016/j.jep.2014.06.042
  12. Ha, S., Vankova, R., Yamaguchi-shinozaki, K., Shinozaki, K., & Tran, L. S. (2012). Cytokinins: metabolism and function in plant adaptation to environmental stresses. Trends in Plant Science, 17 (3), 172-179. doi:https://doi.org/10.1016/j.tplants.2011.12.005
  13. Izquierdo, P. (2006). Development of micropropagation protocols for two species critically endangered Asteraceae endemic of the Galapagos Island. Lyonia, 9 (2), 57-62. Recuperado de https://www.lyonia.org/downloadpdf-2.461.pdf
  14. Jain, A., Pandey, K., Benjamin, D., Meena, A., & Singh, R. (2014). In vitro approach of medicinal herb: Bacopa monnieri. International Journal of Innovative Research in Science, Engineering and Technology, 3 (5), 12088-12093. Recuperado de http://www.ijirset.com/upload/2014/may/12_In-vitro.pdf
  15. Jan, A., Bhat, K.M., Bhat, S.J., Mir, M. A., Bhat, M. A., Imtiyaz, A., &Rather, J. A. (2013). Surface sterilization method for reducing microbial contamination of field grown strawberry explants intended for in vitro culture. African Journal of Biotechnology, 12 (39), 5749-5753. doi:10.5897/AJB2013.12918
  16. Johri, M. M., & Mitra, D. (2001). Action of plant hormones. Current Science, 80 (2), 199-205. Recuperado de https://pdfs.semanticscholar.org/9589/fb99c718faa849b1c96566c3b74875d2c2a9.pdf
  17. Kaur, P., Mall, D., Sheokand, A., Singh, L., & Datta, S. (2018). Role of plant growth regulators in vegetable production: a review. International Journal of Current Microbiology and Applied Sciences, 7 (6), 2177-2183. https://doi.org/10.20546/ijcmas.2018.706.258
  18. Kumar, N., & Reddy, M. P. (2011). In vitro plant propagation: a review. Journal of Forest Science, 27 (2), 61-72. Recuperado de https://www.researchgate.net/publication/263638941_in_vitro_plant_propagation_a_review
  19. Majid, B.N., Roopa, G., Sampath, K.K., Kini, R. K., Prakash, H. S., Abbagani, S., Mehdi, K., & Geetha, N. (2014). Establishment of an efficient explant surface sterilization protocol for in vitro micropropagation of Salacia chinensis L., an endangered anti-diabetic medicinal plant. World Journal of Pharmacy and Pharmaceutical Sciences, 3 (12), 1266-1274. Recuperado de https://www.researchgate.net/publication/317351184_establishment_of_an_efficient_explant_surface_sterilization_protocol_for_in_vitro_micropropagation_of_salacia_chinensis_l_an_endangered_anti-diabetic_medicinal_plant
  20. Mazid, M., Khan, T. A., & Mohammad, F. (2011). Cytokinins, a classical multifaceted hormone in plant system. Journal of Stress Physiology & Biochemistry, 7 (4), 347-368. Recuperado de https://www.researchgate.net/publication/301620057_Cytokinins_A_classical_multifaceted_hormone_in_plant_system
  21. Mng’omba, S. A., Sileshi, G., du Toit, E. S., & Akinnifesi, F. k. (2012). Efficacy and utilization of fungicides and other antibiotics for aseptic plant cultures. In D. Dhanasekaran, N. Thajuddin and A. Panneerselvam (Ed.), Fungicides for Plant and Animal Diseases (pp. 245-254). Croatia: INTECH. doi: 10.5772/1130
  22. Ngomuo, M., Mneney, E., & Ndakidemi, P. (2013). The effects of auxins and cytokinin on growth and development of (musa sp.) var. “yangambi” explants in tissue culture. American Journal of Plant Sciences, 4, 2174–2180. doi: http://dx.doi.org/10.4236/ajps.2013.411269
  23. Niu, S., Li, Z., Yuan, H., Fang, P., Chen, X., & Li, W. (2013). Proper gibberellin localization in vascular tissue is required to regulate adventitious root development in tobacco. Journal of Experimental Botany, 64 (11), 3411-3424. doi:10.1093/jxb/ert186
  24. Olivera, P., Tamariz, C., & Gutiérrez, M. (2010). Desinfección e influencia del bencil aminopurina (BAP) y ácido naftalénacético (ANA) en la multiplicación de Perezia coerulescens Wedd, planta medicinal altoandina. Rev. Aporte Santiaguino, 3 (1), 117-124. Recuperado de http://www.scielo.org.pe/pdf/as/v3n1/a16v3n1.pdf
  25. Olivera-Gonzales, P., Yldefonzo, E., Mestanza, E., & Tamariz-Angeles, C. (2017a). In vitro propagation of Oreocallis grandiflora (Lam.) R. Br., a medicinal threatened plant. Annual Research & Review in Biology, 14 (3), 1-9. doi:https://doi.org/10.9734/ARRB/2017/34724
  26. Olivera-Gonzales, P., Espinoza, R., & Tamariz-angeles, C. (2017b) Multiplicación in vitro y embriogénesis somática de Perezia pinnatifida (Asteraceae) planta medicinal andina, Revista Peruana de Biología, 24 (3), 323-328. doi: http://dx.doi.org/10.15381/rpb.v24i3.13911
  27. Olmos, S., Luciani, G., & Galdeano, E. (2010). Micropropagación. En G. Levitus, V. Echenique, C. Rubinstein, E. Hopp and L. Mroginski Ed.), Biotecnología y mejoramiento vegetal II (pp. 353-362). Argentina: Ediciones INTA. Recuperado de https://www.agroindustria.gob.ar/sitio/areas/escuelagro/_archivos//000011_INTA%20Biotecnologia/000000_Inta%20-%20B%C3%ADotecnolog%C3%ADa.pdf
  28. Padhi, M., & Singh, S. P. (2017). Surface sterilization for reducing microbial contamination in in vitro propagation of lasora (Cordia myxa Roxb.) using nodal segments. International Journal of Current Microbiology and Applied Sciences, 6 (8), 836-842. https://doi.org/10.20546/ijcmas.2017.608.106
  29. Ramírez-Villegas, J., Cuesta, C.F., Devenish, C., & Peralvo, M. (2014). Using species distributions models for designing conservation strategies of Tropical Andean biodiversity under climate change, Journal for Nature Conservation, 22 (5), 391–404. http://dx.doi.org/10.1016/j.jnc.2014.03.007
  30. Roca, W. M., & Mroginski, L. A. (1991). Cultivo de tejidos en la agricultura: fundamentos y aplicaciones. Colombia: Centro Internacional de Agricultura Tropical (CIAT).
  31. Seyyedyousefi, S.R., Kaviani, B., & Dehkaei, N.P. (2013). The effect of different concentrations of NAA and BAP on micropropagation of Alstroemeria. European Journal of Experimental Biology, 3 (5), 133-136. Recuperado de http://www.imedpub.com/articles/the-effect-of-different-concentrations-of-naa-and-bap-on-micropropagation-of-alstroemeria.pdf
  32. Shang, B., Xu, C., Zhang, X., Cao, H., Xin, W., & Hu, Y. (2016). Very-long-chain fatty acids restrict regeneration capacity by confining pericycle competence for callus formation in Arabidopsis. PNAS, 113 (18), 5101-5106. doi:www.pnas.org/cgi/doi/10.1073/pnas.1522466113
  33. Su, Y. H., Liu, Y. B., & Zhang, X. S. (2011). Auxin – cytokinin interaction regulates meristem development. Molecular Plant, 4 (4), 616-625. doi:https://doi.org/10.1093/mp/ssr007
  34. Suárez, O.M., Naranjo, E.J., Garcés, L. A., & Trujillo, S. B. (2011). Organogénesis directa in vitro a partir de hojas de la planta antiplasmodial Solanum nudum Dunal. Revista Colombiana de Biotecnología, 13 (2), 186-192. Recuperado de https://revistas.unal.edu.co/index.php/biotecnologia/article/view/28010/28263
  35. Tamariz-Angeles, C., Olivera-Gonzales, P., & Santillán-Torres, M. (2018). Antimicrobial, antioxidant and phytochemical assessment of wild medicinal plants from Cordillera Blanca (Ancash, Peru). Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, 17 (3), 270-285. Recuperado de https://www.blacpma.usach.cl/sites/blacpma/files/articulo_4_-_1426_-_279_-_285.pdf
  36. Teixeira da Silva, J. A., Kulus, D., Zhang, X., Zeng, S., Ma, G., & Piqueras, A. (2016). Disinfection of explants for saffron (Crocus sativus) tissue culture. Environmental and Experimental Biology, 14, 183–198. doi: 10.22364/eeb.14.25
  37. Tripathi, L., & Tripathi, J. N. (2003). Role of biotechnology in medicinal plants. Tropical Journal of Pharmaceutical Research, 2 (2), 243-253. Recuperado de http://www.bioline.org.br/pdf?pr03018
  38. Umehara, M., Hanada, A., Yoshida, S., Akiyama, K., Arite, T., Takeda-Kamiya, N., Magome, H., Kamiya, Y., Shirasu, K., Yoneyama, K., Kyozuka, J., & Yamaguchi, S. (2008). Inhibition of shoot branching by new terpenoid plant hormones. Nature, 455, 195-200. doi: 10.1038/nature07272
  39. Vieira, E. L., Sousa, G. S., Santos, A. R., & Santos, J. (2010). Manual de fisiologia vegetal. São Luis, Brasil: ediciones EDUFMA. Recuperado de http://www.univale.br/sites/biblioteca/biblioteca_online_agronegocio/livrosbiblioteca/manual%20de%20fisiologia%20vegetal.pdf

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