Revista de Investigaciones Altoandinas - Journal of High Andean Research

Vol. 21 No. 4 (2019)
Original articles

Production of in vitro embryos in vitro a portable carbon dioxide incubator and viability in vivo post transfer to recipients cows

pdf (Spanish)
  • Manuel Guido Pérez Durand,
  • Benicio Quispe Villalta,
  • Uri Harold Perez Guerra
Manuel Guido Pérez Durand
National University of the Altiplano Puno Peru
Benicio Quispe Villalta
National University of the Altiplano Puno Peru
Uri Harold Perez Guerra
National University of the Altiplano Puno Peru

Published 2019-10-25

Keywords

  • fertilization,
  • embryo transfer,
  • pregnancy

How to Cite

Pérez Durand, M. G. ., Quispe Villalta, B. ., & Perez Guerra, U. H. . (2019). Production of in vitro embryos in vitro a portable carbon dioxide incubator and viability in vivo post transfer to recipients cows. Revista De Investigaciones Altoandinas - Journal of High Andean Research, 21(4), 249-256. https://doi.org/10.18271/ria.2019.501

Abstract

The objective was to determine the addition of 5% carbon dioxide (CO2) from effervescent granules or gasified to a portable incubator (taper) to evaluate the in vitro production of embryos of oocytes aspirated from ovaries of cows slaughtered in the slaughterhouse and evaluate viability in vivo through pregnancy in recipient cows. As a portable incubator, a 500 cm3 plastic taper was used, to which a valve for controlling the addition of CO2 was conditioned. The maturation was carried out for 24 h, fertilization for 18 h and embryo culture for 7 d in 500 uL of the media in petry plates and introduced into the portable incubator, which was placed in a water bath at 38.5 ° C. At 72 h post fertilization the proportion of zygotes was determined, at 7 d post culture the embryos were counted. The fertilization results with the addition of CO2 from effervescent granules was 38.28% (103/269), with the addition of gasified CO2 37.24% (146/392) and control 49.82% (144/289) (p<0.05); for embryo production it was 18.95% (51/269), 19.38% (76/392) and 24.56% (71/289), respectively (p>0.05); the pregnancy rate in recipient cows was 38.46% (5/13). In conclusion, it is possible to produce in vitro embryos of cattle, using a portable CO2 incubator at field level.

 

pdf (Spanish)

References

  1. Barceló-Fimbres, M., Campos-Chillón, L. F., Mtango, N. R., Altermatt, J., Bonilla, L., Koppang, R., & Verstegen, J. P. (2015). Improving invitro maturation and pregnancy outcome in cattle using a novel oocyte shipping and maturation system not requiring a CO2 gas phase. Theriogenology, 84(1), 109–117. https://doi.org/10.1016/j.theriogenology.2015.02.020
  2. Dong, Y. J., Bai, X. J., Varisanga, M. D., Mtango, N. R., Otoi, T., Rajamahendran, R., & Suzuki, T. (2004). Production of Cloned Calves by the Transfer of Somatic Cells Derived from Frozen Tissues Using Simple Portable CO2 Incubator. Asian-Australasian Journal of Animal Sciences, 17(2), 168–173. https://doi.org/10.5713/ajas.2004.168
  3. Dong, Y. J., Varisanga, M. D., Mtango, N. R., Aono, M., Otoi, T., & Suzuki, T. (2001). Improvement of the culture conditions for in vitro production of cattle embryos in a portable CO2 incubator. Reproduction in Domestic Animals, 36(6), 313–318. https://doi.org/10.1046/j.1439-0531.2001.00308.x
  4. Fujii, A., Kaedei, Y., Tanihara, F., Ito, A., Hanatate, K., Kikuchi, K., … Otoi, T. (2010). In Vitro Maturation and Development of Porcine Oocytes Cultured in a Straw or Dish Using a Portable Incubator with a CO2 Chamber, 624, 619–624. https://doi.org/10.1111/j.1439-0531.2008.01316.x
  5. Garcia, J. C. V., & Padilla, R. P. (2000). Valores gasometricos estimados para las principales poblaciones y sitios a mayor altitud en Mexico. Revista Del Instituto Nacional de Enfermedades Respiratorias, 13(1), 6–13.
  6. Hamdi, M., Lopera-Vasquez, R., Maillo, V., Sanchez-Calabuig, M. J., Núnez, C., Gutierrez-Adan, A., & Rizos, D. (2018). Bovine oviductal and uterine fluid support in vitro embryo development. Reproduction, Fertility and Development, 30(7), 935–945. https://doi.org/10.1071/RD17286
  7. Hansen, P. J. (2006). Realizing the promise of IVF in cattle — an overview, 65, 119–125. https://doi.org/10.1016/j.theriogenology.2005.09.019
  8. Iwayama, H., Ishikawa, H., & Ohsumi, S. (2005). Attempt at In Vitro Maturation of Minke Whale ( Balaenoptera Bonaerensis ) Oocytes Using a Portable CO2 Incubator. J. Reprod. Dev. 51: 69-75 .
  9. J.J. Parrish, J.L.Susko-PArrish , M.L. Leibfried-Rutltdge, E.S. Crister, W.H. Eyestone, and N. L. F. (1986). Bovine in vitro fertilization with frozen-thawed semen. Theriogenology, Vol. 25(Nro. 4), 951–600.
  10. J.M.Lim; B.C. Reggio; R.A. Godke and W. Hansel. (1999). Development of in-vitro-derived bovine embryos cultured in 5% CO2 in air or in 5% O2, 5% CO2 and 90% N2. Human Reproduction, 14(2), 458–464.
  11. Kandil, O. M., Abdoon, A. S. S., Murakami, M., Otoi, T., & Suzuki, T. (1999). New technique, using a portable co2 incubator, for the production of in vitro fertilized egyptian buffalo embryos. Journal of Reproduction and Development, 45(5), 1–6. https://doi.org/10.1007/978-94-007-2229-3_13
  12. Pontes, J. H. F., Silva, K. C. F., Basso, A. C., Rigo, A. G., & Ferreira, C. R. (2010). Large-scale in vitro embryo production and pregnancy rates from Bos taurus , Bos indicus , and indicus-taurus dairy cows using sexed sperm, 74, 1349–1355. https://doi.org/10.1016/j.theriogenology.2010.06.004
  13. Sanches, B. V., Lunardelli, P. A., Tannura, J. H., Cardoso, L., Colombo, M. H., Gaitkoski, D., … Seneda, M. M. (2016). A new direct transfer protocol for cryopreserved IVF embryos. Theriogenology. https://doi.org/10.1016/j.theriogenology.2015.11.029
  14. Suzuki, T., Sumantri, C., Khan, N. H. A., Murakami, M., & Saha, S. (1999). Development of a simple, portable carbon dioxide incubator for in vitro production of bovine embryos. Animal Reproduction Science, 54(3), 149–157. https://doi.org/10.1016/S0378-4320(98)00134-1
  15. Tervit, H. R., Whittingham, D. G., & April, R. (1972). SUCCESSFUL CULTURE IN VITRO OF SHEEP AND CATTLE OVA Agricultural Research Council , Unit of Reproductive Physiology and Biochemistry , * Physiological Laboratory , University of Cambridge, 493–497.
  16. Tsuchiya, K., Tsuda, S., Kamijima, T., Nishimiya, Y., Aoyagi, Y., & Ideta, A. (2013). A simple medium enables bovine embryos to be held for seven days at 4°C. Scientific Reports, 3(1), 1–5. https://doi.org/10.1038/srep01173
  17. Varisanga, M. D., Dong, Y., Mtango, N. R., Fahrudin, M., Wayan, K. K. N. I., & Suzuki, T. (2000). Bovine blastocyts obtained for reconstructed Cytoplast and Karyoplasts Using a simple portable CO2 incubator. Cloninng, 2(4), 167–173.
  18. Walsh, S. W., Williams, E. J., & Evans, A. C. O. (2011). A review of the causes of poor fertility in high milk producing dairy cows. Animal Reproduction Science, 123(3–4), 127–138. https://doi.org/10.1016/j.anireprosci.2010.12.001
  19. Wilson, R. D., Weigel, K. A., Fricke, P. M., Rutledge, J. J., Matthews, D. L., & Schutzkus, V. R. (2005). In Vitro Production of Holstein Embryos Using Sex-Sorted Sperm and Oocytes from Selected Cull Cows. J. Dairy Sci. 88: 776–782.

Similar Articles

You may also start an advanced similarity search for this article.

Most read articles by the same author(s)