Revista de Investigaciones Altoandinas - Journal of High Andean Research

Vol. 20 Núm. 4 (2018)
Artículo original

Crio-tomografía de rayos X (Cryo-XT) de fábricas virales en células infectadas con Virus vaccinia

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  • José Antonio Moreno-Serrano,
  • Tulio F. Solano Castillo
José Antonio Moreno-Serrano
Universidad Complutense de Madrid España
Tulio F. Solano Castillo
Universidad Complutense de Madrid España

Publicado 2018-10-29

Palabras clave

  • virus vaccinia,
  • cryo-tomografía de rayos X,
  • fábrica viral,
  • filamentos de actina

Cómo citar

Moreno-Serrano, J. A. ., & Solano Castillo, T. F. . (2018). Crio-tomografía de rayos X (Cryo-XT) de fábricas virales en células infectadas con Virus vaccinia. Revista De Investigaciones Altoandinas - Journal of High Andean Research, 20(4), 409-418. https://doi.org/10.18271/ria.2018.418

Resumen

El VV (Vaccinia viurs) es uno de los virus más complejos, con un tamaño superior a 300 nm y más de 100 proteínas estructurales. Su montaje implica interacciones secuenciales y reordenamientos importantes de sus componentes estructurales. Se infectaron las células BSC40 y luego se seleccionaron mediante microscopía de fluorescencia de luz y posteriormente se formaron imágenes en el microscopio de rayos X en condiciones criogénicas. Se utilizaron series de inclinación tomográfica de imágenes de rayos X para producir reconstrucciones tridimensionales que muestran diferentes organelos celulares (núcleos, mitocondrias, RE), junto con otros dos tipos de partículas virales relacionadas con diferentes etapas de maduración del virus vaccinia (IV) inmaduros y (MV) partículas maduras;  los ensayos con witaferina mostraron enlaces con actina, que impide la polimerización y el alargamiento de los filamentos; causando viriones mal empaquetados o aberrantes, que inhibe la progresión de la infección viral. Los hallazgos demuestran que la cryo-tomografía de rayos X, es una poderosa herramienta para recolectar información estructural tridimensional a partir de células enteras congeladas, no fijadas y sin manchas con resolución suficiente para detectar diferentes partículas de virus que exhiben distintos niveles de maduración.

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