Revista de Investigaciones Altoandinas - Journal of High Andean Research

Vol. 25 No. 4 (2023)
Original articles

Antibiotics Effects on biomass, area coverage and chlorophyll of Lemna gibba and Azolla filiculoides

pdf (Spanish)
  • Ingrid Maldonado Jimenez,
  • Jesús Miranda-Mamani,
  • Yesica M. Mamani Arpasi
Ingrid Maldonado Jimenez
Escuela de Posgrado, Universidad Nacional del Altiplano, Puno, Perú
Jesús Miranda-Mamani
Facultad de Ciencias Biológicas. Universidad Nacional del Altiplano, Puno, Perú
Yesica M. Mamani Arpasi
Escuela profesional de Ingeniería Ambiental y Forestal, Universidad Nacional de Juliaca, Puno, Perú

Published 2023-11-04

Keywords

  • area, chlorophyll, area cover, phytoremediation, toxicity

Copyright (c) 2023 Ingrid Maldonado Jimenez, Jesús Miranda-Mamani, Yesica M. Mamani Arpasi

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Maldonado Jimenez, I. ., Miranda-Mamani, J., & Mamani Arpasi, Y. M. (2023). Antibiotics Effects on biomass, area coverage and chlorophyll of Lemna gibba and Azolla filiculoides. Revista De Investigaciones Altoandinas - Journal of High Andean Research, 25(4), 233-240. https://doi.org/10.18271/ria.2023.532

Abstract

The concentrations of antibiotic residues in aquatic ecosystems are experiencing a progressive increase, which is exerting significant impacts on resident species. The species Lemna gibba and Azolla filiculoides are widely known in studies of toxicity and phytoremediation. Furthermore, these species have been observed to exhibit interesting responses to exposure to antibiotic compounds. In this study, the effects of azithromycin, ciprofloxacin, amoxicillin, cephalexin, ampicillin, and clindamycin (0.00, 2.5, 5, 7.5 mg/L) on biomass, Relative Growth Rate (RGR), percentage of area, and chlorophyll were evaluated over a period of 7 days. Biomass data were collected to calculate RGR, and daily images of each experimental unit were captured to estimate the percentage of area coverage and RGB bands for chlorophyll calculation purposes. The results revealed toxic effects of the concentrations compared to the control group, with Azolla showing greater susceptibility than Lemna. Concerning the percentage of area coverage, the experimental days significantly influenced Azolla (p-value = <2e-16), while both the antibiotic concentration and the duration of exposure negatively affected Lemna, with p-values of 0.00161 and 2.1e-11, respectively. As for chlorophyll levels, no significant alterations were detected in response to the evaluated concentrations. These findings demonstrate that despite the adverse effects of antibiotics, the investigated species still exhibit survival and antibiotic removal capabilities.

pdf (Spanish)

References

  1. Ali, M. M., Al-ani, A., Eamus, D., & Tan, D. K. Y. (2012). A New Image Processing Based Technique to Determine Chlorophyll in Plants. American-Eurasian J. Agric. & Environ. Sci., 12(10), 1323-1328. https://doi.org/10.5829/idosi.aejaes.2012.12.10.1917
  2. Baciak, M., Sikorski, Ł., Piotrowicz-Cieślak, A. I., & Adomas, B. (2016). Content of biogenic amines in Lemna minor (common duckweed) growing in medium contaminated with tetracycline. Aquatic Toxicology, 180, 95-102. https://doi.org/10.1016/j.aquatox.2016.09.007
  3. Bianchi, E., Biancalani, A., Berardi, C., Antal, A., Fibbi, D., Coppi, A., Lastrucci, L., Bussotti, N., Colzi, I., Renai, L., Scordo, C., Del Bubba, M., & Gonnelli, C. (2020). Improving the efficiency of wastewater treatment plants: Bio-removal of heavy-metals and pharmaceuticals by Azolla filiculoides and Lemna minuta. Science of the Total Environment, 746, 141219. https://doi.org/10.1016/j.scitotenv.2020.141219
  4. Brain, R. A., Johnson, D. J., Richards, S. M., Hanson, M. L., Sanderson, H., Lam, M. W., Young, C., Mabury, S. A., Sibley, P. K., & Solomon, K. R. (2004). Microcosm evaluation of the effects of an eight pharmaceutical mixture to the aquatic macrophytes Lemna gibba and Myriophyllum sibiricum. Aquatic Toxicology, 70(1), 23-40. https://doi.org/10.1016/j.aquatox.2004.06.011
  5. Brain, R. A., Johnson, D. J., Richards, S. M., Sanderson, H., Sibley, P. K., & Solomon, K. R. (2004). Effects of 25 pharmaceutical compounds to Lemna gibba using a seven-day static-renewal test. Environmental Toxicology and Chemistry, 23(2), 371-382. https://doi.org/10.1897/02-576
  6. Carrapico, F. (2014). Azolla as a Superorganism . Its Implication in Symbiotic Studies. En Symbioses and Stress: Joint Ventures in Biology (pp. 225-241). Springer. https://doi.org/10.1007/978-90-481-9449-0
  7. Costa, C. S., Tetila, E. C., Astolfi, G., Sant’Ana, D. A., Brito Pache, M. C., Gonçalves, A. B., Garcia Zanoni, V. A., Picoli Nucci, H. H., Diemer, O., & Pistori, H. (2019). A computer vision system for oocyte counting using images captured by smartphone. Aquacultural Engineering, 87(September). https://doi.org/10.1016/j.aquaeng.2019.102017
  8. Drobniewska, A., Wójcik, D., Kapłan, M., Adomas, B., Piotrowicz-Cieślak, A., & Nałęcz-Jawecki, G. (2017). Recovery of Lemna minor after exposure to sulfadimethoxine irradiated and non-irradiated in a solar simulator. Environmental Science and Pollution Research, 24(36), 27642-27652. https://doi.org/10.1007/s11356-016-7174-3
  9. Forni, C., Cascone, A., Fiori, M., & Migliore, L. (2002). Sulphadimethoxine and Azolla filiculoides Lam.: A model for drug remediation. Water Research, 36(13), 3398-3403. https://doi.org/10.1016/S0043-1354(02)00015-5
  10. Garcia-Rodríguez, A., Matamoros, V., Fontàs, C., & Salvadó, V. (2015). The influence of Lemna sp. and Spirogyra sp. on the removal of pharmaceuticals and endocrine disruptors in treated wastewaters. International Journal of Environmental Science and Technology, 12(7), 2327-2338. https://doi.org/10.1007/s13762-014-0632-x
  11. Gomes, M. P., de Brito, J. C. M., Carvalho Carneiro, M. M. L., Ribeiro da Cunha, M. R., Garcia, Q. S., & Figueredo, C. C. (2018). Responses of the nitrogen-fixing aquatic fern Azolla to water contaminated with ciprofloxacin: Impacts on biofertilization. Environmental Pollution, 232, 293-299. https://doi.org/10.1016/j.envpol.2017.09.054
  12. Gomes, M. P., Gonçalves, C. A., de Brito, J. C. M., Souza, A. M., da Silva Cruz, F. V., Bicalho, E. M., Figueredo, C. C., & Garcia, Q. S. (2017). Ciprofloxacin induces oxidative stress in duckweed (Lemna minor L.): Implications for energy metabolism and antibiotic-uptake ability. Journal of Hazardous Materials, 328, 140-149. https://doi.org/10.1016/j.jhazmat.2017.01.005
  13. Grenni, P., Patrolecco, L., Rauseo, J., Spataro, F., Di Lenola, M., Aimola, G., Zacchini, M., Pietrini, F., Di Baccio, D., Stanton, I. C., Gaze, W. H., & Barra Caracciolo, A. (2019). Sulfamethoxazole persistence in a river water ecosystem and its effects on the natural microbial community and Lemna minor plant. Microchemical Journal, 149, 103999. https://doi.org/10.1016/j.microc.2019.103999
  14. Hájková, M., Kummerová, M., Zezulka, Š., Babula, P., & Váczi, P. (2019). Diclofenac as an environmental threat: Impact on the photosynthetic processes of Lemna minor chloroplasts. Chemosphere, 224, 892-899. https://doi.org/10.1016/j.chemosphere.2019.02.197
  15. Hattink, J., & Wolterbeek, H. T. (2001). Accumulation of 99Tc in duckweed Lemna minor L. as a function of growth rate and 99Tc concentration. Journal of Environmental Radioactivity, 57(2), 117-138. https://doi.org/10.1016/S0265-931X(01)00015-7
  16. Hu, H., Zhou, Q., Li, X., Lou, W., Du, C., Teng, Q., Zhang, D., Liu, H., Zhong, Y., & Yang, C. (2019). Phytoremediation of anaerobically digested swine wastewater contaminated by oxytetracycline via Lemna aequinoctialis: Nutrient removal, growth characteristics and degradation pathways. Bioresource Technology, 291, 121853. https://doi.org/10.1016/j.biortech.2019.121853
  17. Iatrou, E., Gatidou, G., Damalas, D., Thomaidis, N., & Stasinakis, A. (2017). Fate of antimicrobials in duckweed Lemna minor wastewater treatment systems. Journal of Hazardous Materials, 330, 116-126. https://doi.org/10.1016/j.jhazmat.2017.02.005
  18. Kadir, A. A., Abdullah, S. R. S., Othman, B. A., Hasan, H. A., Othman, A. R., Imron, M. F., Ismail, N. ‘Izzati, & Kurniawan, S. B. (2020). Dual function of Lemna minor and Azolla pinnata as phytoremediator for Palm Oil Mill Effluent and as feedstock. Chemosphere, 259, 1-13. https://doi.org/10.1016/j.chemosphere.2020.127468
  19. Kummerová, M., Zezulka, Š., Babula, P., & Tříska, J. (2016). Possible ecological risk of two pharmaceuticals diclofenac and paracetamol demonstrated on a model plant Lemna minor. Journal of Hazardous Materials, 302, 351-361. https://doi.org/10.1016/j.jhazmat.2015.09.057
  20. Li, D., Li, C., Yao, Y., Li, M., & Liu, L. (2020). Modern imaging techniques in plant nutrition analysis: A review. Computers and Electronics in Agriculture, 174, 1-14. https://doi.org/10.1016/j.compag.2020.105459
  21. Lu, X. M., & Lu, P. Z. (2019). Distribution of antibiotic resistance genes in soil amended using Azolla imbricata and its driving mechanisms. Science of the Total Environment, 692, 422-431. https://doi.org/10.1016/j.scitotenv.2019.07.285
  22. Medina, W., Skurtys, O., & Aguilera, J. M. (2010). Study on image analysis application for identification Quinoa seeds (Chenopodium quinoa Willd) geographical provenance. LWT - Food Science and Technology, 43(2), 238-246. https://doi.org/10.1016/j.lwt.2009.07.010
  23. Minervini, M., Fischbach, A., Scharr, H., & Tsaftaris, S. A. (2016). Finely-grained annotated datasets for image-based plant phenotyping. Pattern Recognition Letters, 81, 80-89. https://doi.org/10.1016/j.patrec.2015.10.013
  24. Nunes, B., Pinto, G., Martins, L., Gonçalves, F., & Antunes, S. C. (2014). Biochemical and standard toxic effects of acetaminophen on the macrophyte species Lemna minor and Lemna gibba. Environmental Science and Pollution Research, 1-8. https://doi.org/10.1007/s11356-014-3059-5
  25. Pomati, F., Netting, A. G., Calamari, D., & Neilan, B. A. (2004). Effects of erythromycin, tetracycline and ibuprofen on the growth of Synechocystis sp. and Lemna minor. Aquatic Toxicology, 67(4), 387-396. https://doi.org/10.1016/j.aquatox.2004.02.001
  26. Pro, J., Ortiz, J. A., Boleas, S., Fernández, C., Carbonell, G., & Tarazona, J. V. (2003). Effect assessment of antimicrobial pharmaceuticals on the aquatic plant Lemna minor. Bulletin of Environmental Contamination and Toxicology, 70(2), 290-295. https://doi.org/10.1007/s00128-002-0208-1
  27. Riccardi, M., Mele, G., Pulvento, C., Lavini, A., D’Andria, R., & Jacobsen, S. E. (2014). Non-destructive evaluation of chlorophyll content in quinoa and amaranth leaves by simple and multiple regression analysis of RGB image components. Photosynthesis Research, 120(3), 263-272. https://doi.org/10.1007/s11120-014-9970-2
  28. Singh, V., Pandey, B., & Suthar, S. (2018). Phytotoxicity of amoxicillin to the duckweed Spirodela polyrhiza: Growth, oxidative stress, biochemical traits and antibiotic degradation. Chemosphere, 201, 492-502. https://doi.org/10.1016/j.chemosphere.2018.03.010
  29. Sree, K. S., Keresztes, Á., Mueller-Roeber, B., Brandt, R., Eberius, M., Fischer, W., & Appenroth, K. J. (2015). Phytotoxicity of cobalt ions on the duckweed Lemna minor – Morphology, ion uptake, and starch accumulation. Chemosphere, 131, 149-156. https://doi.org/10.1016/j.chemosphere.2015.03.008
  30. Taghi ganji, M., Khosravi, M., & Rakhshaee, R. (2005). Biosorption of Pb, Cd, Cu and Zn from the wastewater by treated Azolla filiculoides with H2O2/MgCl2. International Journal of Environmental Science & Technology, 1(4), 265-271. https://doi.org/10.1007/bf03325841
  31. Vannini, A., Paoli, L., Vichi, M., Bačkor, M., Bačkorová, M., & Loppi, S. (2018). Toxicity of Diclofenac in the Fern Azolla filiculoides and the Lichen Xanthoria parietina. Bulletin of Environmental Contamination and Toxicology, 100(3), 430-437. https://doi.org/10.1007/s00128-017-2266-4
  32. Zhu, W., Sun, Z., Yang, T., Li, J., Peng, J., & Zhu, K. (2020). Estimating leaf chlorophyll content of crops via optimal unmanned aerial vehicle hyperspectral data at multi-scales. Computers and Electronics in agriculture, 178, 1-16. https://doi.org/10.1016/j.compag.2020.105786

Similar Articles

You may also start an advanced similarity search for this article.