Effect of NaCl stress on morphological characteristics and SPAD values in Rubus spp. cultivars in vitro
Carlos Eduardo Millones Chanamé- Ernestina Rosario Vásquez Castro
Published 2025-07-17
Keywords
- Salinity,
- growth,
- screening,
- nodal segment,
- in vitro culture
Copyright (c) 2025 Carlos Eduardo Millones Chanamé, Ernestina Rosario Vásquez Castro
This work is licensed under a Creative Commons Attribution 4.0 International License.
How to Cite
Abstract
Salt stress is one of the main problems that restricts crop production, affecting agronomic performance and fruit quality. Therefore, this research was conducted to identify salinity tolerance in Rubus spp. cultivars at the vegetative stage under in vitro conditions. Nodal segments of the three cultivars were placed in growth and development media supplemented with three levels of NaCl (0, 35 and 70 mM). A completely randomized design with a factorial arrangement was used (Factor A: three cultivars and Factor B: two levels of NaCl and control), with three replications. The cultivar ‘Brazos’ registered an adequate response in shoot height when subjected to simulated saline stress with 70 mM NaCl. The cultivars ‘Tupy’ and ‘Brazos’ registered tolerance to 35 mM NaCl in the variable number of leaves compared to the control. The traits number of leaves and shoot height allowed to identify tolerance to saline stress in the vegetative stage of Rubus spp. cultivars under in vitro conditions.