Vol. 18 No. 4 (2016)
Original articles

Phytopathogenic fungi associated with avocado seed (Persea american Mill.)

Betsabe Leon Ttacca
National University of the Altiplano Puno Peru
Leonor Mattos Calderon
National University of the Altiplano Puno Peru

Published 2016-12-20

Keywords

  • Fusarium verticillioides,
  • Lasiodiplodia theobromae,
  • Persea americana,
  • seed

How to Cite

Leon Ttacca, B., & Mattos Calderon, L. . (2016). Phytopathogenic fungi associated with avocado seed (Persea american Mill.). Revista De Investigaciones Altoandinas - Journal of High Andean Research, 18(4), 423-430. https://doi.org/10.18271/ria.2016.216

Abstract

A series of fungi diseases severe is disseminated by avocado seeds and they can decrease the production in approximately 14% and a reduction of quality in 10%. The present investigation consisted in to isolate and identify pathogens fungal present in the avocado seeds mexican race used for patron. The fotopatogenos fungi were isolated in culture medium Sucrose Agar Potato from the embryo and cotyledon of 200 seeds of avocado and to identify with taxonomic keys also was carried out Pathogenicity test on avocado plants with the most frequent phytopathogenic fungi. The pathogens fungal isolated and identified from embryo and cotyledon of seeds were Lasiodiplodia sp. with 0.5% and 1.00%, and Fusarium sp with 1.50% and 2.50% of frecuency respectively. The pathogenicity test allowed confirming that Lasiodiplodia theobromae and Fusarium verticillioides affect to seedlings of avocado, being the source of primary inoculum and means of dissemination infected seeds by these pathogens.

References

  1. Azcon-Bieto, J. 2000. Fundamentos de Fisiología Vegetal. Barcelona, ES. Universidad de Barcelona. 522 p.
  2. Bacon, W.; Yates, E.; Hinton, M.; Meredith, F. 2001. Biological Control of Fusarium moniliforme in Maize. Environmental Helth Pespectives 109 (2): 325-332.
  3. Barnett, HL; and Hunter, BB. 1998. Illustrated genera of imperfect fungi. 3 ed. New York, USA. MacMillan Publishing Company. USA. 218 p.
  4. Barron, GL. 1968. The genera of Hyphomycetes from soil. Baltimore, MD, USA. The Williams and Wilkins Co. 363 p.
  5. Booth, C. 1971. The genus Fusarium. Kew, UK. Commonwealth Mycological Institute. 237 p.
  6. Burges, HD. 1981. Microbial control of pests and plant diseases 1970-1980. London-UK. Academic Press. 949 p.
  7. Cilliers, A. J.; Swart, W. J.; Wingfield, M. J. 1993. A review of Lasiodiplodia theobromae with particular reference to its occurrence on coniferous seeds. Suid-Afrikaaense Bosboutydskrif 166: 47-52Coutinho W. M.; Pereira L. A.; Machado J. C.; Freitas-Silva O.; Pena R. C.; Magalhaes, F. H. 2000. Effects of sodium hypochlorite on the conidial germination of some seed-borne fungi. Fitopatologia Brasileira, 25(3):552-555.
  8. Cedeño, L; Carreño, C; Mohali, S; Palacios-Pru, E; Quintero, K. 1995. Muerte regresiva de parchita causada por Lasiodiplodia theobromae en Venezuela. Fitopatología Venezolana 8 (1): 11-14.
  9. Coutinho, WM; Pereira, LA; Machado, JC; Freitas-Silva, O; Pena, RC; and Magalhaes, FH. 2000. Effects of sodium hypochlorite on the conidial germination of some seed-borne fungi. Fitopatologia Brasileira 25(3):552-555.
  10. Fisher, N; Burgess, L; Toussoun, T; Nelson, P. 1982. Carnation leaves as a substrate and for preserving cultures of Fusarium species. Phytopathology 72: 151-153
  11. Joffe, A. 1972. Fusaria isolated from avocado, banana and citrus fruit in Israel and their Pathogenicity. Plant Disease Reporter 56 (11): 963-966.
  12. Joffe, A; Palti, J; Arbel-Sherman, R.1973. Fusarium moniliforme Sheld. in Israel (Gibberella Fujikuroi (Saw.) Wollenw.). Mycophatologia applicata. 50(2): 85-107.
  13. Lager, J; Johnsson, L. 2002. Seedborne fungi affect field emergence in red clover. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz 109(4):350-356.
  14. Leal-Nares, O; Gonzales, E; Vidales, I. 2004. Embriogenesis somática por el cultivo in Vitro de tejido nucelar de frutos inmaduros de aguacate (Persea americana Mill.) cv. Hass. Morelia, MX. Instituto de investigaciones Químico-Biológicas, UMSNH. 8 p.
  15. Leslie, JF; Summerell, BA. 2006. The Fusarium Laboratory Manual. Ames, USA. Blackwell Publishing. 388 p.
  16. Ministerio de Agricultura, 2008. Estudio de palta en el Perú y el Mundo. Dirección General de Información Agraria, 23 p.
  17. Mont Koc, R. 2000. El palto y sus enfermedades. Lima, PE. SENASA. 48 p.
  18. Nagerabi, SA; Elshafie, AE. 2001. Determination of seedborne fungi and aflatoxins in Sudanese guar seeds. Tropical Science 41(1):31-35.
  19. Nelson, P; Toussoun, T; Cook, R. 1981. Fusarium: Diseases, biology and taxonomy. Pennsylvania, USA. The Pennsylvania State University Press. 457 p.
  20. Nelson, P; Toussoun, T; Marasas, W. 1983. Fusarium species: An illustrated manual for identification. Pennsylvania, USA. The Pennsylvania State University Press. 193 p.
  21. Oliveira EC; Andrade, GA. 1996. Occurrence and survival of Botryodiplodia theobromae Pat. in cotton seeds (Gossypium hirsutum L.) produced in the state of Minas Gerais, Brazil. Ciencia e Agrotecnologia 20:31-38.
  22. Ouf, SA. 1993. Mycoflora and population dynamics of some seed borne fungi in relation to the fungicide Benlate. Zentralblatt fur Mikrobiologie 148(8):570-581.
  23. Química Suiza, 2005. Promet Cu: Inductor de fenolasas. Ficha técnica. Lima, PE. 5 p.
  24. Reddy, VK; Reddy, SM; Reddy, SM; Srivastava, HP. 1997. Biochemical changes in developing kernels of maize under the influence of Lasiodiplodia theobromae. Microbial Biotechnology 183-186.
  25. Seifert, K. A.; Aoki, T.; Baayen, R. P.; Brayford, D.; Burgess, L. W.; Chulze, S.; Gams, W.; Geiser, D. ; De Gruyter, D., J. ; Leslie, J. F. ; Logrieco, A. ; Marasas, W. F. O.; Nirenberg, H. I.; Donnell, K. O; Rheeder, J. P.; Samuels, G. J.; Summerell, B. A.; Thrane, U.; Waalwijk, C. 2003. The name Fusarium moniliforme should no longer be used. Mycological Research 107: 643-644. (8, 9, verticillioides)
  26. Sutton, B. 1980. The Coelomycetes: Fungi imperfecti with pycnidia, acervuli and stromata. Surrey, England. Commonwealth Mycological Institute. 697 p.
  27. Tousson, TA; Nelson, P.E. 1976. Fusarium. A pictorial guide to the identification of Fusarium species according to the taxonomic system of Snyder and Hansen. 2 ed. University Park y Londres: The Pennsylvania State University Press. 43 p.