Vol. 19 No. 1 (2017)
Original articles

Prospecting of phytoparasitic nematodes in potato crops in the Puno region

Yeni Yeni Flores-Choque
National University of the Altiplano Puno Peru
Israel Lima Medina
National University of the Altiplano Puno Peru
Rosario Bravo Portocarrero
National University of the Altiplano Puno Peru

Published 2017-03-30

Keywords

  • Morphological characterizatión,
  • Biochemical characterizatión

How to Cite

Flores-Choque, Y. Y. ., Lima Medina, I. ., & Bravo Portocarrero, R. . (2017). Prospecting of phytoparasitic nematodes in potato crops in the Puno region. Revista De Investigaciones Altoandinas - Journal of High Andean Research, 19(1), 11-20. https://doi.org/10.18271/ria.2017.251

Abstract

The presence of plant-parasitic nematodes in agricultural crops causes large losses in production worldwide, up to 12%. In the Puno region the nematodes are causing damage to the potato crop; Being the objective of the present work to identify plant parasitic nematodes in potato culture (Solanum tuberosum L.) of the Puno region. 160 samples from seven provinces of the Puno region were evaluated during the 2015-2016. The collected soil samples were processed by the method of centrifugal fluctuation in sucrose solution, subsequently identified genres using a stereoscope, based in morphological and biochemical characterization by electrophoresis, respectively. The results showed the presence of eight genero of plant-parasitic nematodes: Meloidogyne, Nacobbus, Pratylenchus, Helicotylenchus, Xiphinema, Dorylaimus, Globodera and free living nematodes (considered saprophagus and predatory nematodes).

References

  1. Agrios, G. (2011). Fitopatología. México: Editorial Limusa S.A. de CV Grupo Noriega Editores, 745 – 749.
  2. Ahmad, W. y Jairajpuri, M. S. (1982): Opisthodorylaimus n. gen, and some new and known species of Dorylaimoidea (Nematoda) from India. Révue du Nématologie, 5: 261–275.
  3. Carneiro, R. y Almeida, M. (2001). Técnica de eletroforese usada no estudo de enzimas dos nematoides das galhas para identificação de espécies. Nematologia Brasileira 25, 35 – 44.
  4. Di Vito, M., Greco, N., Carputo, D. y Frusciante, L. (2003). Respuesta de clones de papa silvestres y cultivadas a poblaciones Italianas del nematodo agallador Meloidogyne spp. Nematropica v 33 n 1, 65 – 72.
  5. Doucet, E. M. y Di Rienzo, A. J. (1991). Caracterización morfológica y morfométrica de poblaciones de N. aberrans (Thorne, 1935) Throne y allen, 1944. Laboratorio de nematología, centro de zoología aplicada, universidad de Córdoba, Argentina. Nematrópica Vol. 21 No 1.
  6. DRA, Dirección Regional Agraria (2016). Oficina de Estadística Agraria e Informática. Recuperado de: http://www.agropuno.gob.pe/?q=node/1022#punto
  7. Eisenback, J. D. y Triantaphyllou, H. H. (1991). Root-knot nematode: Meloidogyne spp. and races. In: Nickle, W.R. (ed.) Manual of agricultural nematology. Marcel Dekker New York. 191 – 174.
  8. Ferris, H. (1999). Nematode Plant Expert Information System (NEMAPLEX). University of California. Recuperado de: http://plpnemweb.ucdavis.edu/nemaplex/index.htm
  9. Freitas, L. G., Neves, W. S. y Olivera, R. D. (2007). Métodos en nematologia vegetal. In: Alfenas, A. C., Mafia, R. G. metodos en fitopatología. Editora de la Universidad federal de Visosa. Vicosa. Minas gerais, 253 – 292.
  10. Gonzaga, V. (2006). Caracterização morfológica, morfométrica e multiplicação in vitro das seis espécies mais comuns de Pratylenchus Filipjev, 1936 que ocorrem no Brasil. Universidade Estadual Paulista Câmpus De Jaboticabal Faculdade De Ciências Agrárias E Veterinárias – Brasil, 94.
  11. Hunt, D., y Handoo, Z. (2009). Taxonomy, identification and principal species. In Perry, R.; Moens, M; Starr, J. eds. Root-knot nematodes. London, UK. CAB International, 55 – 88.
  12. INIA, Instituto Nacional de Innovación Agraria (2012). Producción de tubérculos semillas de buena calidad de papa. Programa nacional de innovación agraria en raíces y tuberosas. Serie: manual N° 01-2012 Puno - Perú.
  13. Jenkins, W. (1964). A rapid centrifugal-flotation technique for separating nematodes from soil. Plant disease reporter, v. 48, 692.
  14. Kaushal, K. K. (2013). Plant nematology - Cyst and root knot nematodes. BIOTECH BOOKS. New Delhi, 331.
  15. Karssen, G. y Moens, M. (2006). Taxonomy and Principal General Root-Knot Nematodes. En: Plant Nematology (Perry, R. y Moens, M. Eds). CAB International, Wallingford, UK. Part I, Chapter 3, 60-90.
  16. Koenning, R. y Barker, K. R. (1998). Developing sustainable systems for nematode management. Annual Review Phytopathology 36,165 – 205.
  17. Lima, M. I., Bellé, C., Casa, C. V. H., Da Silva, P. A. y Bauer, G. C. (2016). Reação de cultivares de batata aos nematoides-das-galhas. Programa de Pós-Graduação em Fitossanidade, Universidade Federal de Pelotas - Brasil.
  18. Mai, W. y Mullin, P. (1996). Plant parasitic nematodes Apictorict key to geners. Fifth edition. Comstock Publishing Associates a Division of Cornell University Press, 277.
  19. Mulvey, R. H. y Anderson, R. V. (1979). Benthic species of Dorylaimus Dujardin, 1845 (nematode: Dorylaimidae) and Arctidorylaimus n. gen. from the Mackenzietories, Canada. Can, J. Zool., 57, 743 – 755.
  20. Ortuño, N., Rojas, B., Oros, R. y Díaz, O. (2013). Meloidogyne sp. atacando el cultivo de papa en zonas altas y frías de Bolivia, 70.
  21. Perry, R. y Moens, M. (2013). Plant Nematology 2nd edition. Printed and bound by Gutenberg Press Ltd, Tarxien, Malta, 542.
  22. Perry, R, Moens, M. y Starr, J. (2009). Root-knot Nematodes. UK by the MPG Books Group. 530, 31.
  23. Pinheiro, J. B. y Lopes, C. A. (2011). Manejo integrado de nematoides em cultivos de batata In: ZAMBOLIM, L. Produção integrada da batata. Volume 2. Viçosa: Universo Agrícola, 69 – 94.
  24. Sen, D., Chatterjee, A. y Manna, B. (2011). A new species of dorylaimus dujardin, 1845 (nematoda: dorylaimidae) from west bengal, India Nematol. medit, 39: 3-8
  25. Sher, S.A. (1970). Revision of the genus Nacobbus Thorne and Allen 1944 (Nematoda: Tylenchoidea). Journal of Nematology 2: 228–235.
  26. Subbotin, S.A., Mundo-Ocampo, M. y Baldwin, J.G. (2010). Systematics of cyst nematodes (Nematoda: Heteroderinae). Nematology Monographs and Perspectives 8A (Series Editors: Hunt, D.J. & Perry, R.N.). Leiden, The Netherlands, Brill, 351.
  27. Taylor, A. L. y Sasser, J. N. (1983). Biología, identificación y control de los nematodos de nódulo de la raíz (especies de Meloidogyne). Proyecto internacional de Meloidogyne, 111.
  28. Tihohod, D. (2000). Nematología Agrícola Aplicada. Jaboticabal-Brasil. FAPESP, 472.
  29. Trudgill, D. L. y Blok, V. C. (2001). Apomictic polyphagous root-knot nematodes: exepcionally successful and damaging biotrophic root pathogens. Ann. Rev. Phytopathol 39, 53 - 77. Recuperado de: http://www.monografias.com/trabajos75 /nematodos-fitoparasitos-manejo-formadores-agallas/nematodos-fitoparasitos-manejo-formadores-agallas2.shtml#ixzz4LUdQWCoy