Revista de Investigaciones Altoandinas - Journal of High Andean Research

Vol. 22 No. 3 (2020)
Original articles

Isolation of bacteria with bioremediation potential and analysis of bacterial communities in an area affected by an oil spill in Condorcanqui (Amazonas, Peru)

pdf (Spanish)
  • Rosita T. Castillo Rogel,
  • Francis J. More Calero,
  • Melitza Cornejo La Torre,
  • Jaime N. Fernández Ponce,
  • Eric L. Mialhe Matonnier
Rosita T. Castillo Rogel
Facultad de Ciencias, Universidad Nacional de Piura, Peru
Bio
Francis J. More Calero
Empresa de investigación y capacitación en Biotecnología Molecular, IncaBiotec, Tumbes, Peru
Melitza Cornejo La Torre
Cooperativa de trabajadores Biotecoop, Lima, Peru
Jaime N. Fernández Ponce
Facultad de Ciencias, Universidad Nacional de Piura, Peru
Eric L. Mialhe Matonnier
Empresa de investigación y capacitación en Biotecnología Molecular, IncaBiotec, Tumbes, Peru

Published 2020-09-04

Keywords

  • water,
  • bacteria,
  • hydrocarbons,
  • metagenomics,
  • soil

Copyright (c) 2020 Rosita T. Castillo Rogel, Francis J. More Calero, Melitza Cornejo La Torre, Jaime N. Fernández Ponce, Eric L. Mialhe Matonnier

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Castillo Rogel, R. T., More Calero, F. J., Cornejo La Torre, M., Fernández Ponce, J. N. ., & Mialhe Matonnier, E. L. . (2020). Isolation of bacteria with bioremediation potential and analysis of bacterial communities in an area affected by an oil spill in Condorcanqui (Amazonas, Peru). Revista De Investigaciones Altoandinas - Journal of High Andean Research, 22(3), 215-225. https://doi.org/10.18271/ria.2020.656

Abstract

The use of oil and its derivatives has been intensified by the current high energy demand, but brought with it the increase in environmental accidents such as fuel spills that negatively affect ecosystems. In those environments there are microorganisms capable of surviving such conditions and using petroleum hydrocarbons as a source of carbon and energy; those can be used in bioremediation, with an eco-friendly and cost-effective approach. In this work, bacterial strains with bioremediation potential were isolated and identified in culture media supplemented with oil from an area contaminated from an oil spill in the Peruvian Amazon; also was done out the characterization of the bacterial community   by independent analysis of culture by means of next generation sequencing directed to the 16S rRNA gene. The isolated bacterial strains were identified as Acinetobacter rudis, Enterobacter cloacae, Klebsiella oxytoca, Morganella morganii, Proteus hauseri, Proteus terrae, Proteus vulgaris (2), Pseudomonas koreensis, Pseudomonas moraviensis, Pseudomonas prosekii y Serratia marcescens (2). The culture-independent analysis detected the Proteobacteria and Bacteroidetes phylum as predominant in water and soil contaminated with hydrocarbons; Likewise, the taxonomic allocation at the family level highlighted the groups Flavobacteriaceae, Moraxellaceae, Verrucomicrobia and Acetobacteraceae as the most abundant, in addition to the genera Acinetobacter, Flavobacterium and Geobacter present in both samples. In this way, the main groups involved in the degradation of petroleum hydrocarbons were determined using culture-dependent and independent techniques.

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