Vol. 20 Núm. 2 (2018)
Artículo original

Contenido de aminas biógenas y calidad microbiológica del charqui de alpaca

Bettit K. Salvá
Universidad Nacional Agraria La Molina, Lima – Perú
Javier Mateo
Universidad de León, España

Publicado 2018-04-27

Palabras clave

  • charqui,
  • alpaca,
  • aminas biógenas,
  • microbiología,
  • carne seca

Cómo citar

Salvá, B. K. ., & Mateo, J. . (2018). Contenido de aminas biógenas y calidad microbiológica del charqui de alpaca. Revista De Investigaciones Altoandinas - Journal of High Andean Research, 20(2), 179-188. https://doi.org/10.18271/ria.2018.362

Resumen

Se analizó el contenido de aminas biógenas, así como la flora aerobia mesófila viable,  Staphylococcus aureusMicrococcaceae, coliformes, mohos y levaduras y bacterias ácido lácticas en 52 muestras de charqui de alpaca (30 muestras de charqui deshilachado y 22 muestras de charqui entero con hueso). Se observaron diferencias significativas en la carga microbiana entre el charqui deshilachado y entero, encontrándose mayores recuentos microbiológicos en el charqui deshilachado. En cuanto al contenido de aminas biógenas se encontraron, respectivamente, un promedio total de 131,60 y 92,30 mg/kg de charqui. Las aminas biógenas mayoritarias encontradas en el charqui deshilachado fueron espermina (52,06 mg/kg), triptamina+ 2-feniletilamina (28,75 mg/kg), tiramina (16,61 mg/kg) y putrescina (12,49 mg/kg), mientras que en el charqui entero las aminas biógenas mayoritarias fueron espermina (39,72 mg/kg), cadaverina (14,75 mg/kg), tiramina (14,71 mg/kg) y triptamina+feniletilamina (12,18 mg/kg). No obstante a esas diferencias, la presencia de aminas biógenas en ambos tipos de charqui estuvieron por debajo de los valores considerados como indicativos de actividad microbiana indeseable. Además, ninguna de las muestras de charqui analizadas tuvo un contenido de aminas biógenas vasoactivas (tiramina, histamina, triptamina y 2-feniletilamina) potencialmente perjudicial para la salud del consumidor, ya que no sobrepasaron los límites considerados como tóxicos en los alimentos.

Referencias

  1. Ansorena, D., Montel, M.C., Rokka, M., Talon, R., Eerola, S., Rizzo, A., Raemaekers, M., & Demeyer, D. (2002). Analysis of biogenic amines in northern and southern European sausages and role of flora in amine production. Meat Science, 61(2), 141-147.
  2. Bennani, L., Zenati, Y., Faid, M., & Ettayebi, M. (1995). Physico-chemical and microbiological characteristics of a dried salted meat product (Kaddid) in Morocco. Lebensmittel-Untersuchung und-Forschung, 201(6), 528-532.
  3. Biscola, V., Abriouel, H., Todorov, S. D., Capuano, V. S., Gálvez, A., & Franco, B. D. G. (2014). Effect of autochthonous bacteriocin-producing Lactococcus lactis on bacterial population dynamics and growth of halotolerant bacteria in Brazilian charqui. Food Microbiology, 44, 296-301.
  4. Chukwu, O., & Imodiboh, L.I. (2009). Influence of storage conditions on shelf-life of dried beef product (Kilishi). World Journal of Agricultural Sciences, 5(1), 34-39.
  5. Cordero, M. R., & Zumalacárregui, J. M. (2000). Characterization of Micrococcaceae isolated from salt used for Spanish dry-cured ham. Letters in Applied Microbiology, 31(4), 303-306.
  6. Downes, F. P. (Ed.). (2001). Compendium of Methods for the Microbiological Examination of Foods (4a ed.). Washington, DC: American Public Health Association.
  7. Eerola, H. S., Sagués, A. R., & Hirvi, T. K. (1998). Biogenic amines in finnish dry sausages. Journal of Food Safety, 18(2), 127-138.
  8. Eerola, S., Hinkkanen, R., Lindfors, E., & Hirvi, T. (1993). Liquid chromatographic determination of biogenic amines in dry sausages. Journal of AOAC International, 76(3), 875-877.
  9. Galgano, F., Favati, F., Bonadio, M., Lorusso, V., & Romano, P. (2009). Role of biogenic amines as index of freshness in beef meat packed with different biopolymeric materials. Food Research International, 42 (8), 1147-1152.
  10. García, I., Zumalacárregui, J. M., & Díez, V. (1995). Microbial succession and identification of Micrococcaceae in dried beef cecina, an intermediate moisture meat product. Food Microbiology, 12, 309-315.
  11. Halász, A., Baráth, A., Simon-Sarkadi, L., & Holzapfel, W. (1994). Biogenic amines and their production by microorganisms in food. Trends in Food Science and Technology, 5(2), 42-49.
  12. Hernández-Jover, T., Izquierdo-Pulido, M., Veciana-Nogués, M. T., Mariné-Font, A., & Vidal-Carou, M. C. (1997). Biogenic amine and polyamine contents in meat and meat products. Journal of Agricultural and Food Chemistry, 45(6), 2098-2102.
  13. Instituto Nacional de Defensa de la Competencia y de la protección de la Propiedad Intelectual. (2006). Norma Técnica Peruana 201.059: 2006. Carne y productos cárnicos. Charqui. Requisitos. Lima. Perú.
  14. International Organization for Standardization. (1995). Norma ISO 13681. Meat and meat products - Enumeration of yeasts and moulds - Colony-count technique.
  15. International Organization for Standardization. (2003a). Norma ISO 6887-2. Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 2: Specific rules for the preparation of meat and meat products.
  16. International Organization for Standardization. (2003b). Norma ISO 4833. Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of microorganisms - Colony-count technique at 30 degrees C.
  17. International Organization for Standardization. (2003c). Norma ISO 6888-3. Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 3: Detection and MPN technique for low numbers.
  18. Kaban, G. (2009). Changes in the composition of volatile compounds and in microbiological and physicochemical parameters during pastirma processing. Meat Science, 82(1), 17-23.
  19. Kilic, B. (2009). Current trends in traditional Turkish meat products and cuisine. LWT-Food Science and Technology, 42(10), 1581-1589.
  20. Lara, J. A. F., Senigalia, S. W. B., Oliveira, T. C. R. M., Dutra, I. D. S., Pinto, M. F., & Shimokomaki, M. (2003). Evaluation of survival of Staphylococcus aureus and Clostridium botulinum in charqui meats. Meat Science, 65(1), 609-613.
  21. Lorenzo, J. M., Munekata, P. E. S., & Domínguez, R. (2017). Role of autochthonous starter cultures in the reduction of biogenic amines in traditional meat products. Current Opinion in Food Science, 14, 61–65.
  22. Mamani-Linares, W., & Cayo, F. (2011). Características físico-químicas del charqui de llama. Revista de Investigaciones Veterinarias del Perú, 22(4), 290-300.
  23. Molinero, C., Martínez, B., Rubio, B., Rovira, J., & Jaime, I. (2008). The effects of extended curing on the microbiological, physicochemical and sensorial characteristics of Cecina de León. Meat Science, 80(2), 370-379.
  24. Nortjé, K., Buys, E. M., & Minnaar, A. (2005). Effect of γ-irradiation on the sensory quality of moist beef biltong. Meat Science, 71(4), 603-611.
  25. Paleari, M. A., Bersani, C., Vittorio, M. M., & Beretta, G. (2002). Effect of curing and fermentation on the microflora of meat of various animal species. Food Control, 13(3), 195-197.
  26. Papageorgiou, M., Lambropoulou, D., Morrison, C., Kłodzińska, E., Namieśnik, J., & Płotka-Wasylka, J. (2018). Literature update of analytical methods for biogenic amines determination in food and beverages. Trends in Analytical Chemistry, 98, 128-142.
  27. Petit, T., Caro, Y., Petit, A. S., Santchurn; S. J., & Collignan, A. (2014). Physicochemical and microbiological characteristics of biltong, a traditional salted dried meat of South Africa. Meat Science, 96(3), 1313–1317.
  28. Prior, B. A. (1984). Rol of micro-organisms in biltong flavour development. Journal of Applied Bacteriology, 56(1), 41-45
  29. Rubio, B., Martínez, B., García-Cachan, M.D., Rovira, J. & Jaime, I. (2007). Effect of high pressure preservation on the quality of dry cured beef "Cecina de León". Innovative Food Science & Emerging Technologies, 8(1), 102-110.
  30. Salvá, B. K., Fernández-Diez, A., Ramos, D. D., Caro, I., & Mateo, J. (2012). Chemical composition of alpaca (Vicugna pacos) charqui. Food chemistry, 130(2), 329-334.
  31. Santos, M. H. (1996). Biogenic amines: their importance in foods. International Journal of Food Microbiology, 29(2-3), 213-231.
  32. Shalaby, A R. (1996). Significance of biogenic amines to food safety and human health. Food Research International, 29(7), 675-690.
  33. Shalaby, A. R. (1993). Survey on biogenic amines in Egyptian foods: Sausage. Journal of the Science of Food and Agriculture, 62(3), 291–293.
  34. Suzzi, G., & Gardini, F. (2003). Biogenic amines in dry fermented sausages: a review. International Journal of Food Microbiology, 88(1), 41-54.
  35. Ten Brink, B., Damink, C., & Joosten, H. M. L. J. (1990). Occurrence and formation of biologically active amines in foods. International Journal of Food Microbiology, 11(1), 73-84.
  36. Torres, E. A. F. S., Shimokomaki, M., Franco, B. D. G. M., Landgraf, M., Carvalho, Jr. B. C., & Santos, J. C. (1994). Parameters determining the quality of charqui, an intermediate moisture meat product. Meat Science, 38(2), 229-234.
  37. Treviño, E., Beil, D., & Steinhart, H. (1997). Determination of biogenic amines in mini-salami during long-term storage. Food Chemistry, 58(4), 385-390.
  38. Vidal-Carou, M. C., Izquierdo-Pulido, M. L., Martin-Morro, M. C., & Font, M. (1990). Histamine and tyramine in meat products: Relationship with Meat Spoilage. Food Chemistry, 37(4), 239-249.
  39. Vinci, G., & Antonelli, M. L. (2002). Biogenic amines: quality index of freshness in red and white meat. Food Control, 13(8), 519–524.
  40. Virgili, R., Saccani, G., Gabba, L., Tanzi, E., & Bordini, C. S. (2007). Changes of free amino acids and biogenic amines during extended ageing of Italian dry-cured ham. LWT-Food Science and Technology, 40, 871–878.
  41. Yalçın, M. Y., & Șeker, M. (2016). Effect of salt and moisture content reduction on physical and microbiological properties of salted, pressed and freeze dried turkey meat. LWT-Food Science and Technology, 68, 153-159