Revista de Investigaciones Altoandinas - Journal of High Andean Research

Vol. 20 No. 4 (2018)
Original articles

Molecular identification of lactic acid bacteria with probiotic properties isolated from the posterior intestine of Nile tilapia (Orechromis niloticus)

pdf (Spanish)
  • Arnaldo E. Castañeda,
  • Jorge L. Aguilar,
  • Adrian E. Zatan,
  • Odalis E. Toledo,
  • Manuel A. Feria,
  • Deysy Castillo
Arnaldo E. Castañeda
Research and training company in molecular biotechnology , Incabiotec - Tumbes, Peru
Jorge L. Aguilar
Research and training company in molecular biotechnology , Incabiotec - Tumbes, Peru
Adrian E. Zatan
Research and training company in molecular biotechnology , Incabiotec - Tumbes, Peru
Odalis E. Toledo
Research and training company in molecular biotechnology , Incabiotec - Tumbes, Peru
Manuel A. Feria
Research and training company in molecular biotechnology , Incabiotec - Tumbes, Peru
Deysy Castillo
Research and training company in molecular biotechnology , Incabiotec - Tumbes, Peru

Published 2018-12-29

Keywords

  • lactic acid bacteria,
  • probiotics,
  • molecular identification,
  • intestine,
  • tilapia

How to Cite

Castañeda, A. E. ., Aguilar, J. L. ., Zatan, A. E. ., Toledo, O. E. ., Feria, M. A. ., & Castillo, D. . (2018). Molecular identification of lactic acid bacteria with probiotic properties isolated from the posterior intestine of Nile tilapia (Orechromis niloticus). Revista De Investigaciones Altoandinas - Journal of High Andean Research, 20(4), 429-438. https://doi.org/10.18271/ria.2018.420

Abstract

Probiotic bacteria in aquaculture represent a control alternative and prevention of pathogens, in addition, reduce the use of antibiotics. However, it is necessary to search for other groups of bacteria with similar or better properties, obtained even from the same source. The aim of the study was to identify molecularly lactic acid bacteria with probiotic properties isolated from the posterior intestine of Nile tilapia (Oreochromis niloticus). The study was carried out from November 2017 to March 2018 evaluating the antagonistic, proteolytic, amylolytic, and antibiotic sensitivity activities, and antagonistic to the following pathogens: Aeromonas hydrophila, Aeromonas veronii, Pseudomonas aeruginosa, Pseudomonas putida and Plesiomonas shigelloides. It was identified by sequencing the 16S rRNA gene that Enterococcus faecium (IP5-2a) and Pediococcus pentosaceus (IP5-2b) inhibited the growth of pathogens, in addition, were sensitive to the antibiotics chloramphenicol, amoxicillin/clavulanic acid, gentamicin, erythromycin and tetracycline, respectively. The results show that E. faecium and P. pentosaceus possess the best antagonistic activities, property of greater relevance to determine a potentially probiotic bacterium. Therefore, it is concluded that the bacteria identified in the present study are good candidates to be considered probiotic, however, it is necessary to confirm their in vivo properties.

pdf (Spanish)

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