Revista de Investigaciones Altoandinas - Journal of High Andean Research

Vol. 22 No. 2 (2020)
Case report

Anti-Trichophyton rubrum activity of the essential oil of Clinopodium brevicalyx and elaboration of a topical emulsion

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  • Cinthya Merma Ccana,
  • Ciro Ciro Tomaylla Cruz,
  • Carla del Carpio Jiménez
Cinthya Merma Ccana
Escuela Profesional de Farmacia y Bioquímica, Universidad Nacional de San Antonio Abad del Cusco, Perú
Ciro Ciro Tomaylla Cruz
Escuela Profesional de Química, Universidad Nacional de San Antonio Abad del Cusco, Perú
Carla del Carpio Jiménez
Laboratorio de Tecnología Farmacéutica, Escuela Profesional de Farmacia y Bioquímica, Universidad Nacional de San Antonio Abad del Cusco, Perú

Published 2020-08-30

Keywords

  • Chemical composition,
  • Gas chromatography-mass espectrometry,
  • agar diffusion,
  • broth macrodilution

Copyright (c) 2020 Cinthya Merma Ccana, Ciro Tomaylla Cruz and Carla del Carpio Jiménez

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Merma Ccana, C., Ciro Tomaylla Cruz, C., & del Carpio Jiménez, C. (2020). Anti-Trichophyton rubrum activity of the essential oil of Clinopodium brevicalyx and elaboration of a topical emulsion. Revista De Investigaciones Altoandinas - Journal of High Andean Research, 22(2), 182-190. https://doi.org/10.18271/ria.2020.606

Abstract

Clinopodium brevicalyx grows in the southern high Andean regions of Peru and has wide popular use, however to date there is no research on its activity against Trichophyton rubrum, an anthropophilic dermatophyte fungus with an affinity for human keratin and difficult to treat, causing the most common superficial mycoses such as athlete's foot, ringworm and nail infections. The aim of this research was to determine the activity of the essential oil of Clinopodium brevicalyx against Trichophyton rubrum ATCC 28188. The essential oil was obtained by hydrodistillation, the extraction rate was 1.98 ± 0.02% (w/v), and the physicochemical characteristics were: relative density of 0.93654 g/mL, a refractive index of 1.4625, an optical rotation of 2.14°, and pH of 6.20. The chemical composition of the essential oil was determined using a gas chromatograph coupled to a mass spectrometer, identifying as main components: isomentone (44.24 %), menthone (22.22 %) and pulegone (8.23 %). The sensitivity of T. rubrum was evaluated by the disk diffusion method, establishing that at higher oil concentration more sensitivity of the fungus, the minimum inhibitory concentration of the oil was determined by the broth macro-dilution, obtaining a minimum inhibitory concentration (MIC) of 125 μL/mL. The topical emulsion with different percentages of essential oil, showed antifungal activity since 12.5% using the macrodilution method. Concluding that both the essential oil and the emulsion at 12.5% have antifungal activity against Trichophyton rubrum.

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